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PECAM-1 Antibody [C31.7]PECAM-1 Antibody [C31.7]PECAM-1 Antibody [C31.7]PECAM-1 Antibody [C31.7]PECAM-1 Antibody [C31.7]PECAM-1 Antibody [C31.7]
PECAM-1 Antibody [C31.7]
PECAM-1 Antibody [C31.7]
PECAM-1 Antibody [C31.7]
PECAM-1 Antibody [C31.7]
PECAM-1 Antibody [C31.7]
PECAM-1 Antibody [C31.7]

PECAM-1 Antibody [C31.7]

CATALOG NUMBER: 33-185
  • Clonality: Monoclonal
  • Tested Applications: Flow, IF, IHC, WB
  • Host Species: Mouse
  • Species Reactivity: Cynomolgus Monkey, Human, Rabbit
  • Conjugate: Unconjugated
  • Size: 100 ug

Specifications

  • HOST SPECIES: Mouse
  • SPECIES REACTIVITY: Cynomolgus Monkey, Human, Rabbit
  • IMMUNOGEN: Human recombinant PECAM-1 / CD31 protein was used as the immunogen for this antibody.
  • CONJUGATE: Unconjugated
  • TESTED APPLICATIONS: Flow, IF, IHC, WB
  • APPLICATION NOTE: Flow Cytometry: 0.5-1ug/10e6 cells
    IF: 1-2 ug/ml
    WB: 0.5-1 ug/ml
    IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
    Prediluted format : incubate for 30 min at RT (2)
    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the PECAM-1 antibody to be titered up or down for optimal performance.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Properties

  • PURIFICATION: Protein G affinity chromatography
  • CLONALITY: Monoclonal
  • ISOTYPE: IgG1, kappa
  • PHYSICAL STATE: Liquid
  • BUFFER: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
  • CONCENTRATION: 0.2 mg/mL
  • STORAGE CONDITIONS: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.

Additional Info

  • NCBI OFFICIAL SYMBOL: PECAM1
  • ADDITIONAL NAMES: Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, CD31, PECAM1
  • NCBI GENE ID NUMBER: 5175
  • USER NOTE: Optimal dilutions for each application to be determined by the researcher

Background

  • PECAM-1, or platelet endothelial cell adhesion molecule-1, also called CD31, is made up of six extracellular immunoglobulin-like domains, a single transmembrane domain, and a cytoplasmic domain containing serine and tyrosine residues that when phosphorylated, activate the molecules signaling function. It is expressed on platelets, monocytes, neutrophils, bone marrow macrophage and dendritic cells precursors, and at the intracellular junction of endothelial cells. PECAM-1, on the endothelial lining of blood vessels, helps maintain the vascular barrier. In response to inflammatory mediators, the endothelial pull away from each other allowing fluid, inflammatory cells and proteins into the extravascular space. The inflammatory response fades as adhesion of neighboring cells increase. PECAM-1 also plays an important role in angiogenesis, promoting endothelial cell motility, junctional development and capillary morphogenesis.

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER: 33-185

  • Size: 100 ug
  • List Price: $555.00
Datasheet
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