Specifications
- HOST SPECIES: Mouse
- SPECIES REACTIVITY: Human
- IMMUNOGEN: Mouse monoclonal PD-L2 antibodies were raised against the extracellular domain of human PD-L2.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, Flow, ICC, IF, IHC-P, WB
- SET CONTENTS: PD-L2 Antibody [4E10], Cat. No. RF16021 (20 μg)
PD-L2 Antibody [8C12], Cat. No. RF16022 (20 μg)
PD-L2 Antibody [7C7], Cat. No. RF16023 (20 μg)
PD-L2 Antibody [7C1], Cat. No. RF16024 (20 μg)
PD-L2 Antibody [10H4], Cat. No. RF16025 (20 μg) - POSITIVE CONTROL 1: Cat. No. RF10001-06 - Raji Lysate
- POSITIVE CONTROL 2: Cat. No. RF10001-04 - Human Tonsil Tissue Lysate
Properties
- PURIFICATION: Antibodies are supplied as protein A purified IgG1.
- CLONALITY: Monoclonal
- ISOTYPE: IgG1
- PHYSICAL STATE: Liquid
- BUFFER: PBS containing 0.02% sodium azide.
- CONCENTRATION: Antibody 1 mg/mL
- STORAGE CONDITIONS: Antibodies can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PD-L1 and PD-L2, both of which are thought act as a negative regulator of T cell activation. However, it has been suggested that PD-L2 can act to stimulate an immunogenic response through an alternative receptor from PD-1.ProSci's Risk-FreeTM antibodies are mouse monoclonal antibodies made to improve in vivo studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-FreeTM antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Validated for flow cytometry and ELISA Rigorously tested for the following applications: Immunoblot Immunohistochemistry Immunocytochemistry Immunofluorescence Multiple antibodies per target allowing the user to choose the best antibody for their application Available individually or as a set Risk-FreeTM means they are guaranteed to work for their approved applications
- 1: Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.
- 2: Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.
- 3: LaGier J and Pober JS. Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1). Hum. Immunol. 2006; 67:568-78.
- 4: Zhang Y, Chung Y, Bishop C, et al. Regulation of T cell activation and tolerance by PDL2. Proc. Natl. Acad. Sci. USA 2006; 103:11695-700.
Set Contents
- Catalog Number RF16021: PDL2 Antibody [4E10]
- Catalog Number RF16022: PDL2 Antibody [8C12]
- Catalog Number RF16023: PDL2 Antibody [7C7]
- Catalog Number RF16024: PDL2 Antibody [7C1]
- Catalog Number RF16025: PDL2 Antibody [10H6]
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: This product is for research use only.
Shipping Info
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