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- SPECIES: Mouse
- SOURCE SPECIES: HEK293 cells
- RECOMBINANT PROTEIN SEQUENCE: Phe 19 - Thr 238
- FUSION TAG: His Tag
- TESTED APPLICATIONS: WB
- APPLICATION NOTE: This recombinant protein can be used for WB. For research use only.
- PREDICTED MOLECULAR WEIGHT: 25.6 kDa
- BIOLOGICAL ACTIVITY: Measured by its binding ability in a functional ELISA. Immobilized Mouse PD-L1, His Tag at 10 ug/mL (100 ul/well) can bind Mouse PD-1, Fc Tag with a linear range of 0.16-5 ug/mL.
Properties
- PURITY: >95% as determined by SDS-PAGE.
- PHYSICAL STATE: Lyophilized
- BUFFER: PBS, pH7.4
- STORAGE CONDITIONS: Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: Cd274
- ADDITIONAL NAMES: PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1
- Protein Accession Number: NP_068693
- NCBI GENE ID NUMBER: 60533
Background
- Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.
- 1: Dong H., et al., 1999, Nat. Med. 5:1365-1369.
- 2: Freeman G.J., et al., 2000, J. Exp. Med. 192:1027-1034.
- 3: Nishimura H., et al., 2001, Trends in Immunology 22:265.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.
Shipping Info
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