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PDL1 Antibody [1F11]
PDL1 Antibody [1F11]
PDL1 Antibody [1F11]
PDL1 Antibody [1F11]
PDL1 Antibody [1F11]
PDL1 Antibody [1F11]
PDL1 Antibody [1F11]
  • Trial Size

PDL1 Antibody [1F11]

CATALOG NUMBER: RF16037
  • Clonality: Monoclonal
  • Tested Applications: ELISA, ICC, IF, IHC-P, WB
  • Host Species: Mouse
  • Species Reactivity: Human
  • Conjugate: Unconjugated
  • Size: 0.02 mg | 0.1 mg

Specifications

  • HOST SPECIES: Mouse
  • SPECIES REACTIVITY: Human
  • IMMUNOGEN: PD-L1 antibody was raised against the extracellular domain of human PD-L1.
  • CONJUGATE: Unconjugated
  • TESTED APPLICATIONS: ELISA, ICC, IF, IHC-P, WB
  • APPLICATION NOTE: PD-L1 antibody can be used for detection of PD-L1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 - μg/mL. For immunofluorescence start at 5 μg/mL.
    Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
  • POSITIVE CONTROL 1: Cat. No. 1340 - Human Stomach Carcinoma Tissue Lysate
  • POSITIVE CONTROL 2: Cat. No. 1315 - Human Tonsil Tissue
  • PREDICTED MOLECULAR WEIGHT: Predicted: 32 kDa
    Observed: 45 kDa

Properties

  • PURIFICATION: PD-L1 Antibody is supplied as protein A purified IgG1.
  • CLONALITY: Monoclonal
  • ISOTYPE: IgG1
  • PHYSICAL STATE: Liquid
  • BUFFER: PD-L1 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
  • CONCENTRATION: 1 mg/mL
  • STORAGE CONDITIONS: PD-L1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

  • NCBI OFFICIAL SYMBOL: CD274
  • ADDITIONAL NAMES: PD-L1 Antibody: Programmed cell death 1 ligand-1, programmed death ligand 1, PDL1, PDL-1, B7-H1
  • Protein Accession Number: NP_054862
  • PROTEIN GI NUMBER: 7661534
  • NCBI GENE ID NUMBER: 29126
  • USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background

  • PD-L1 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC) (1). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC (2). PD-1 can bind two different but related ligands, PD-L1 and PD-L2. PD-L1 is a B7-related protein that inhibits cell-mediated immune responses by reducing the secretion of IL-2 and IL-10 from memory T cells (3). This suggests that PD-L1 may be useful in reducing allogenic CD4+ memory T-cell responses to endothelial cells, thereby reducing the likelihood of host immune responses to allografts. PD-L1 also functions as an immune checkpoint protein, and multiple anti-PD-L1 antibodies are currently in phase II and III clinical trials, with one antibody already approved for the treatment of cancer (4).
  • 1: Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.
  • 2: Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.
  • 3: LaGier J and Pober JS. Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1). Hum. Immunol. 2006; 67:568-78.
  • 4: Aydin AM, Woldu SL, Hutchinson RC, et al. Spotlight on atezolizumab and its potential in the treatment of advanced urothelial bladder cancer.Onco. Targets Ther. 2017;10:1487-502.

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER: RF16037

  • Size: 0.02 mg | 0.1 mg
  • List Price: $89.00 – $459.00
Datasheet
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