- Trial Size
PDGF-B Antibody
CATALOG NUMBER: 7199- Clonality: Polyclonal
- Tested Applications: E, IF, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Sheep: (100%)
- IMMUNOGEN: Rabbit polyclonal PDGF-B antibody was raised against a 19 amino acid peptide near the carboxy terminus of human PDGF-B.
The immunogen is located within the last 50 amino acids of PDGF-B. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, WB
- APPLICATION NOTE: PDGF-B antibody can be used for detection of PDGF-B by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in rat samples and Immunofluorescence in human samples. All other applications and species not yet tested. - POSITIVE CONTROL 1: Cat. No. 1464 - Rat Liver Tissue Lysate
- POSITIVE CONTROL 2: Cat. No. 10-201 - Human Liver Tissue Slide
- PREDICTED MOLECULAR WEIGHT: Predicted: 27 kDa
Properties
- PURIFICATION: PDGF-B Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: PDGF-B Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: PDGF-B antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Additional Info
- NCBI OFFICIAL SYMBOL: PDGFB
- ADDITIONAL NAMES: PDGF-B Antibody: SIS, SSV, IBGC5, PDGF2, c-sis, PDGF-2, SIS, Platelet-derived growth factor subunit B, PDGF subunit B
- Protein Accession Number: NP_002599
- PROTEIN GI NUMBER: 4505681
- NCBI GENE ID NUMBER: 5155
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- PDGF-B Antibody: The platelet-derived growth factor beta (PDGF-B) is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB). Mutations in this gene are associated with meningioma , and reciprocal translocations between chromosomes 22 and 7, at sites where the PDGF-B gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans.
- 1: Johnsson A, Heldin CH, Wasteson A, et al. The c-sis gene encodes a precursor of the B chain of platelet-derived growth factor. EMBO J. 1984; 3:921-8.
- 2: Fredriksson L, Li H, and Eriksson U. The PDGF family: four gene products for five dimeric isoforms. Cyto. Growth Factor Rev. 2004; 15:197-204.
- 3: Maxwell M, Galanopoulos T, Hedley-Whyte ET, et al. Human meningiomas co-express platelet-derived growth factor )PDGF) and PDGF-receptor genes and their protein products. Int. J. Cancer 1990; 46:16-21.
- 4: Simon MP, Navarro M, Roux D, et al. Structural and functional analysis of a chimeric protein COL1A1-PDGFB generated by the translocation t(17;22)(q22;q13.1) in Dermatofibrosarcoma protuberans (DP). Oncogene 2001; 20:2965-75.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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$35 Dry Ice Shipment fee may be required on some items.
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