PDF Antibody
CATALOG NUMBER: 60-274- Clonality: Polyclonal
- Tested Applications: WB
- Host Species: Rabbit
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 400 ul
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- IMMUNOGEN: This PDF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 125-151 amino acids from the Central region of human PDF.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: WB
- APPLICATION NOTE: For WB starting dilution is: 1:1000
- PREDICTED MOLECULAR WEIGHT: 27 kDa
Properties
- PURIFICATION: This antibody is purified through a protein A column, followed by peptide affinity purification.
- CLONALITY: Polyclonal
- ISOTYPE: Rabbit Ig
- PHYSICAL STATE: Liquid
- BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: PDF
- ADDITIONAL NAMES: Peptide deformylase, mitochondrial, Polypeptide deformylase, PDF, PDF1A
- Protein Accession Number: Q9HBH1
- PROTEIN GI NUMBER: 17433054
- NCBI GENE ID NUMBER: 64146
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Protein synthesis proceeds after formylation of methionineby methionyl-tRNA formyl transferase (FMT) and transfer of thecharged initiator f-met tRNA to the ribosome. In eubacteria andeukaryotic organelles the product of this gene, peptide deformylase(PDF), removes the formyl group from the initiating methionine ofnascent peptides. In eubacteria, deformylation of nascent peptidesis required for subsequent cleavage of initiating methionines bymethionine aminopeptidase. The discovery that a natural inhibitorof PDF, actinonin, acts as an antimicrobial agent in some bacteriahas spurred intensive research into the design ofbacterial-specific PDF inhibitors. In human cells, onlymitochondrial proteins have N-formylation of initiatingmethionines. Protein inhibitors of PDF or siRNAs of PDF block thegrowth of cancer cell lines but have no effect on normal cellgrowth. In humans, PDF function may therefore be restricted torapidly growing cells.
- 1: Escobar-Alvarez, S., et al. J. Mol. Biol. 387(5):1211-1228(2009)
- 2: Wang, L., et al. Cancer Epidemiol. Biomarkers Prev. 17(12):3558-3566(2008)
- 3: Lee, M.D., et al. J. Clin. Invest. 114(8):1107-1116(2004)
- 4: Serero, A., et al. J. Biol. Chem. 278(52):52953-52963(2003)
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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$35 Dry Ice Shipment fee may be required on some items.
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