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- SPECIES: Rabbit
- SOURCE SPECIES: HEK293 cells
- RECOMBINANT PROTEIN SEQUENCE: Leu 25 - Gln 167
- FUSION TAG: Fc Tag
- TESTED APPLICATIONS: WB
- APPLICATION NOTE: This recombinant protein can be used for WB. For research use only.
- PREDICTED MOLECULAR WEIGHT: 42.6 kDa
Properties
- PURITY: >95% as determined by SDS-PAGE.
Endotoxin level is less than 1.0 EU per ug by the LAL method. - PHYSICAL STATE: Lyophilized
- BUFFER: Tris with Glycine, Arginine and NaCl, pH7.5
- STORAGE CONDITIONS: Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: PD-1
- ADDITIONAL NAMES: PDCD1,PD1,CD279,SLEB2
- Protein Accession Number: G1SUF0
- NCBI GENE ID NUMBER: 100343779
Background
- Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-gammaby suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-gammasecretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.
- 1: Ishida Y., et al., 1992, EMBO J. 11 (11): 3887–95.
- 2: Blank C., et al., 2007, Cancer Immunol. Immunother. 56 (5): 739–45.
- 3: Agata Y., et al., 1996, Int. Immunol. 8 (5): 765–72.
- 4: Freeman GJ., et al., 2000, J. Exp. Med. 192 (7): 1027–34.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.
Shipping Info
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