Specifications
- SPECIES: Mouse
- SOURCE SPECIES: HEK293 cells
- RECOMBINANT PROTEIN SEQUENCE: Gln 35 - Gln 694
- FUSION TAG: His Tag
- TESTED APPLICATIONS: WB
- APPLICATION NOTE: This recombinant protein can be used for WB. For research use only.
- PREDICTED MOLECULAR WEIGHT: 72 kDa
- BIOLOGICAL ACTIVITY: Measured by its binding ability in a functional ELISA Immobilized mouse PCSK9 at 10μg/ml (100μl/well) can bind biotinylated rhLDLR. The EC50 of biotinylated human LDLR is 0. 1μg/ml.
Properties
- PURITY: >97% as determined by SDS-PAGE.
- PHYSICAL STATE: Lyophilized
- BUFFER: PBS, pH7.4
- STORAGE CONDITIONS: Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: Pcsk9
- ADDITIONAL NAMES: PCSK9, FH3, HCHOLA3, LDLCQ1, NARC1, PC9
- Protein Accession Number: AAH38085.1
- NCBI GENE ID NUMBER: 100102
Background
- Proprotein convertase subtilisin/kexin type 9 (PCSK9) is also known as NARC1 (neural apoptosis regulated convertase), is a newly identified subtilase belonging to the peptidase S8 subfamily. Mouse PCSK9 is synthesized as a soluble zymogen, and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted active enzyme with a broad alkaline pH optimum. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).
- 1: Seidah NG, et al., Proc. Natl. Acad. Sci. U.S.A. 100 (3): 928–33.
- 2: Abifadel, M. et al., 2003, Nat. Genet. 34: 154-156.
- 3: Dubuc G. et al., 2004, Arterioscler. Thromb. Vasc. Biol. 24 (8): 1454–9.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.
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