- Trial Size
PCDH12 Antibody
CATALOG NUMBER: 5071- Clonality: Polyclonal
- Tested Applications: ELISA, IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: PCDH12 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human PCDH12.
The immunogen is located within amino acids 30 - 80 of PCDH12. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, IHC-P, WB
- APPLICATION NOTE: PCDH12 antibody can be used for detection of PCDH12 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested. - SPECIFICITY: This antibody is predicted to not cross-react with PCDH18.
- POSITIVE CONTROL 1: Cat. No. 1204 - K562 Cell Lysate
- PREDICTED MOLECULAR WEIGHT: Predicted: 128 kDa
Observed: 125 kDa
Properties
- PURIFICATION: PCDH12 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: PCDH12 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: PCDH12 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: PCDH12
- ADDITIONAL NAMES: PCDH12 Antibody: VECAD2, VE-cadherin-2, Protocadherin-12, Vascular cadherin-2, VE-cad-2
- Protein Accession Number: NP_057664
- PROTEIN GI NUMBER: 7706113
- NCBI GENE ID NUMBER: 51294
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- PCDH12 Antibody: Protocadherins comprise the largest group within the cadherin family of calcium-dependent cell-cell adhesion molecules. Protocadherin 12 (PCDH12) was initially identified through PCR screening of mouse heart microvascular endothelial cell RNA; further experiments revealed its mRNA to be strongly expressed in highly vascularized organs such as lung and kidney, in addition to glycogen-rich trophoblasts in the placenta. PCDH12-null mice are viable and fertile, but show reduced placental and embryonic sizes when compared to wild-type mice. Further studies showed significant expression changes in 2, 289 genes, including those involved in tissue morphogenesis, angiogenesis, cell-matrix adhesion and migration, immune response and chromatin remodeling.
- 1: Frank M and Kemler R. Protocadherins. Curr. Opin. Cell Biol.2002; 14:557-62.
- 2: Telo P, Breviaro F, Huber P, et al. Identification of a novel cadherin (vascular endothelial cadherin-2) located at intercellular junctions in endothelial cells. J. Biol. Chem.1998; 28:17565-72.
- 3: Rampon C, Prandini MH, Bouillot S, et al. Protocadherin 12 (VE-cadherin 2) is expressed in endothelial, trophoblast, and mesangial cells. Exp. Cell Res.2005; 302:48-60.
- 4: Rampon C, Bouillot S, Climescu-Haulica A, et al. Protocadherin 12 deficiency alters morphogenesis and transcriptional profile of the placenta. Physiol. Genomics2008; 34:193-204.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
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