PAX3 Antibody
CATALOG NUMBER: 46-138- Clonality: Polyclonal
- Tested Applications: ELISA, IHC, WB
- Host Species: Goat
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 0.1 mg
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- HOST SPECIES: Goat
- SPECIES REACTIVITY: Human
- HOMOLOGY: Expected Species Reactivity based on sequence homology: Rat, Cow
- IMMUNOGEN: The immunogen for this antibody is: TTLAGAVPRMM-C
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, IHC, WB
- APPLICATION NOTE: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 45kDa band observed in lysates of Human Duodenum, Skin, and in lysates of cell lines A431 (calculated MW of 45.2kDa according to NP_852125.1 and of 44.8kDa according to NP_852126.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Esophagus expression of PAX3 is detected in squamous epithelium. Recommended concentration: 4.5ug/ml.
- SPECIFICITY: This antibody is expected to recognize all 8 reported isoforms (NP_852122.1; NP_000429.2; NP_039230.1; NP_852123.1; NP_852124.1; NP_852126.1; NP_852125.1; NP_001120838.1).
- POSITIVE CONTROL 1: Cat. No. 1365 - Human Esophagus Tissue Lysate
Properties
- PURIFICATION: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
- CONCENTRATION: 500 ug/mL
- STORAGE CONDITIONS: Aliquot and store at -20°C. Minimize freezing and thawing.
Additional Info
- NCBI OFFICIAL SYMBOL: PAX3
- ADDITIONAL NAMES: PAX3, WS1, CDHS, HUP2, paired box gene 3 (Waardenburg syndrome 1), paired domain gene 3, paired box homeotic gene 3 (Waardenburg syndrome 1), paired box 3, MGC120381, MGC120382, MGC120383, MGC120384, MGC134778, paired box homeotic gene 3, paired domain gene HuP2
- Protein Accession Number: NP_852122.1,NP_000429.2,NP_039230.1,NP_852123.1,NP_852124.1,NP_852126.1,NP_852125.1,NP_001120838.1
- PROTEIN GI NUMBER: 6654638
- NCBI GENE ID NUMBER: 5077
Background
- 1: Tsukamoto K, Nakamura Y, Niikawa N. Isolation of two isoforms of the PAX3 gene transcripts and their tissue-specific alternative expression in human adult tissues. Hum Genet. 1994 Mar;93(3):270-4.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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