Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- IMMUNOGEN: p56Dok-2 (Phospho-Tyr299) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 299 (G-E-Y (p) -A-V) derived from Human p56Dok-2.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: IF, IHC, WB
- APPLICATION NOTE: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
- SPECIFICITY: This antibody detects endogenous level of p56Dok-2 only when phosphorylated at tyrosine 299.
- PREDICTED MOLECULAR WEIGHT: 56 kDa
Properties
- PURIFICATION: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: Store antibody at -20°C for up to one year.
Additional Info
- NCBI OFFICIAL SYMBOL: DOK2
- ADDITIONAL NAMES: p56DOK, p56dok-2, CPP32, DOK2
- Protein Accession Number: NP_003965.2
- PROTEIN GI NUMBER: 41406050
- NCBI GENE ID NUMBER: 9046
Background
- DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation
- 1: Feng Cong, et, al. (1999) Mol. Cell. Biol ; 19: 8314 - 8325. Serge Lemay, et, al. (2000) Mol. Cell. Biol ; 20: 2743 - 2754. Ute Schaeper, et, al. (2000) J. Cell Biol ; 149: 1419. Miyuki Honma, et, al. (2006) Genes Cells; 11: 143 - 151.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.