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p53 Antibody [PAb 122]
CATALOG NUMBER: 33-854- Clonality: Monoclonal
- Tested Applications: Flow, IF, IHC-Fr, WB
- Host Species: Mouse
- Species Reactivity: Dog, Hamster, Human, Monkey, Mouse, Rat
- Conjugate: Unconjugated
- Size: 100 ug
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- HOST SPECIES: Mouse
- SPECIES REACTIVITY: Dog, Hamster, Human, Monkey, Mouse, Rat
- IMMUNOGEN: SV40-transformed mouse B4 cells were used as the immunogen for this antibody. Its epitope maps near the C-terminal end (aa 370-378) of p53.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: Flow, IF, IHC-Fr, WB
- APPLICATION NOTE: Western blot: 0.5-1 ug/ml
Flow Cytometry: 0.5-1 ug/million cells
Immunofluorescence: 0.5-1 ug/ml
IHC (Frozen) only: 0.5-1 ug/ml for 30 min at RT
Variations in protocols, secondaries and substrates may require the p53 antibody to be titered up or down for optimal performance.
Properties
- PURIFICATION: Protein G affinity chromatography
- CLONALITY: Monoclonal
- ISOTYPE: IgG2b, kappa
- PHYSICAL STATE: Liquid
- BUFFER: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
- CONCENTRATION: 0.2 mg/mL
- STORAGE CONDITIONS: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: TP53
- ADDITIONAL NAMES: Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53, P53
- NCBI GENE ID NUMBER: 7157
- USER NOTE: Optimal dilutions for each application to be determined by the researcher
Background
- This antibody is specific to a conserved determinant of the p53 protein. PAb 122 binds to the C-terminus (aa 370-378) of both wild type and mutated p53. When microinjected into nuclei, PAb 122 blocked re-entry into the S-phase of the cell cycle. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in families. p53 binds to a DNA consensus sequence, the p53 response element, and it regulates normal cell growth cycle events by activating transcription of genes, involved either in progression through the cycle, or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 5-1000 fold over the minute concentrations (1000 molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min). The protein in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. Its the most commonly mutated gene in spontaneously occurring human cancers. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dermal fibroblasts following mild ultraviolet irradiation.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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