Specifications
- SPECIES REACTIVITY: Human
- IMMUNOGEN: Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins.
- TESTED APPLICATIONS: IF, WB
- SET CONTENTS: ORAI1 Antibody, Catalog No. 4041 (50 μg)
ORAI2 Antibody, Catalog No. 4111 (50 μg)
ORAI3 Antibody (CT), Catalog No. 4215 (50 μg)
STIM1 Antibody, Catalog No. 4119 (50 μg)
STIM2 Antibody (CT), Catalog No. 4123 (50 μg)
Properties
- PURIFICATION: Antibodies are supplied as affinity chromatography purified IgG.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: PBS containing 0.02% sodium azide.
- CONCENTRATION: Antibody 1 mg/mL
- STORAGE CONDITIONS: Stable at 4°C for three months, store at -20°C for up to one year.
Additional Info
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. The ORAI family is a recently identified set of proteins that are essential components of these CRAC channels. A missense mutation in the ORAI1 protein in humans is the cause of one form of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with the stromal interaction molecule 1 (STIM1), the store-activated endoplasmic reticulum Ca++ sensor. Like ORAI1, ORAI2 also functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, although at a lesser efficacy than ORAI1. Although ORAI3 can also function as Ca++ plasma membrane channel, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter. STIM1, in its function as a Ca++ sensor and an activator of CRAC channels, migrates to the plasma membrane from endoplasmic reticulum-like sites which act as cellular Ca++ stores. A related molecule, STIM2, inhibits the STIM1-mediated store-operated Ca++ entry, and can form complexes with STIM1, suggesting these two proteins may play a coordinated role in controlling Ca++ entry. The ORAI antibodies are predicted to have no cross-reactivity to the other ORAI proteins. Similarly, the STIM antibodies will not cross-react with the other STIM protein.
For images please see PDF data sheet - 1: Lewis RS. Calcium signaling mechanisms in T lymphocytes. Annu. Rev. Immunol. 2001; 19:497-521.
- 2: Luik RM and Lewis RS. New insights into the molecular mechanisms of store-operated Ca2+ signaling in T cells. Trends Mol. Med. 2007; 13:103-7.
- 3: Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85.
- 4: Soboloff J, Spassova MA, Dziadek MA, et al. Calcium signals mediated by STIM and Orai proteins - a new paradigm in inter-organelle communication. Biochim. Biophys. Acta. 2006; 1763:1161-8.
Set Contents
- Catalog Number 4041: ORAI1 Antibody
- Catalog Number 4111: ORAI2 Antibody
- Catalog Number 4215: ORAI3 Antibody
- Catalog Number 4119: STIM1 Antibody
- Catalog Number 4123: STIM2 Antibody
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: This product is for research use only.
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