Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- IMMUNOGEN: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NUP50.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, WB
- APPLICATION NOTE: NUP50 antibody can be used for detection of NUP50 by ELISA at 1:312500. NUP50 antibody can be used for detection of NUP50 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
- POSITIVE CONTROL 1: Cat. No. 1211 - HepG2 Cell Lysate
- PREDICTED MOLECULAR WEIGHT: 50 kDa
Properties
- PURIFICATION: Antibody is purified by peptide affinity chromatography method.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: For short periods of storage (days) store at 4°C. For longer periods of storage, store NUP50 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: NUP50
- ADDITIONAL NAMES: NUP50, MGC39961, NPAP60, NPAP60L
- Protein Accession Number: NP_009103
- PROTEIN GI NUMBER: 24497451
- NCBI GENE ID NUMBER: 10762
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. NUP50 is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import.The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene.
- 1: Olsen, J.V., (2006) Cell 127 (3), 635-648.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: This product is for research use only.
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