Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: NUP160 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human NUP160.
The immunogen is located within the last 50 amino acids of NUP160. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, IHC-P, WB
- APPLICATION NOTE: NUP160 antibody can be used for detection of NUP160 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. - POSITIVE CONTROL 1: Cat. No. 1203 - A549 Cell Lysate
- POSITIVE CONTROL 2: Cat. No. 10-301 - Human Brain Tissue Slide
Properties
- PURIFICATION: NUP160 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: NUP160 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: NUP160 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: NUP160
- ADDITIONAL NAMES: NUP160 Antibody: KIAA0197, NUP120, Nuclear pore complex protein Nup160, 160 kDa nucleoporin
- Protein Accession Number: EAW67878.1
- PROTEIN GI NUMBER: 119588287
- NCBI GENE ID NUMBER: 23279
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- NUP160 Antibody: The nuclear pore complex (NPC) is a protein assembly localized at the nuclear rim and mediates macromolecular transport between the nucleus and the cytoplasm. The mammalian nucleoporin (NUP)-160 is part of the hetero-oligomeric complex that also contains NUP107, NUP133, NUP96, and mammalian homolog of yeast sec13p. While the majority of the NUP107-160 nuclear pore sub-complex localizes to the nuclear pore, a small fraction is observed at kinetochores and pro-metaphase spindle poles in mitotic cells in association with proteins such as Mad1, Mad2, Bub3 and Cdc20. Immunodepletion of the NUP107-160 complex resulted in defective spindle assembly indicating that it has multiple functions. NUP160 has recently been identified as an HIV dependency factor (HDF), suggesting that NUP160 may be an important drug target in HIV treatment. Multiple isoforms of NUP160 are known to exist.
- 1: Tran EJ and Wente SR. Dynamic nuclear pore complex: life on the edge. Cell2006; 125:1041-53.
- 2: Boehmer T, Enninga J, Dales S, et al. Depletion of a single nucleoporin, Nup107, prevents the assembly of a subset of nucleoporins into the nuclear pore complex. Proc. Natl. Acad. Sci. USA2003; 100:981-5.
- 3: Orjalo AV, Arnaoutov A, Shen Z, et al. The Nup107-160 nucleoporin complex is required for correct bipolar spindle assembly. Mol. Bio. Cell2006; 17:3806-18.
- 4: Brass AL, Dykxhoorn DM, Benita Y, et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science2008; 319:921-6.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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