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Home / Flow / Nucleoli Marker Antibody [NCM102-1]
Nucleoli Marker Antibody [NCM102-1]Nucleoli Marker Antibody [NCM102-1]Nucleoli Marker Antibody [NCM102-1]
Nucleoli Marker Antibody [NCM102-1]
Nucleoli Marker Antibody [NCM102-1]
Nucleoli Marker Antibody [NCM102-1]

Nucleoli Marker Antibody [NCM102-1]

CATALOG NUMBER: 33-890
  • Clonality: Monoclonal
  • Tested Applications: Flow, ICC, IF, IHC
  • Host Species: Mouse
  • Species Reactivity: Human
  • Conjugate: Unconjugated
  • Size: 100 ug

Specifications

  • HOST SPECIES: Mouse
  • SPECIES REACTIVITY: Human
  • IMMUNOGEN: A preparation of myeloid leukemia cell nuclei was used as the immunogen.
  • CONJUGATE: Unconjugated
  • TESTED APPLICATIONS: Flow, ICC, IF, IHC
  • APPLICATION NOTE: Flow Cytometry: 0.5-1ug/10^6 cells
    IF: 0.5-1.0 ug/ml
    Immunocytochemistry (Acetone-fixed): 0.5-1.0 ug/ml for 30 min at RT
    IHC (Formalin-paraffin): 0.5-1.0 ug/ml for 30 min at RT (1)
    Prediluted format : incubate for 30 min at RT (2)
    Titering of the Nucleoli marker antibody may be required for optimal performance.

    1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Properties

  • PURIFICATION: Protein G affinity chromatography
  • CLONALITY: Monoclonal
  • ISOTYPE: IgG1, kappa
  • PHYSICAL STATE: Liquid
  • BUFFER: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
  • CONCENTRATION: 0.2 mg/mL
  • STORAGE CONDITIONS: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.

Additional Info

  • NCBI GENE ID NUMBER: Unknown
  • USER NOTE: Optimal dilutions for each application to be determined by the researcher

Background

  • The nucleolus is the largest structure in the nucleus of eukaryotic cells, where it primarily serves as the site of ribosome synthesis and assembly. Nucleoli also have other important functions like assembly of signal recognition particles and playing a role in the cell's response to stress. Nucleoli are made of proteins and RNA and form around specific chromosomal regions. [Wiki]

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER: 33-890

  • Size: 100 ug
  • List Price: $535.00
Datasheet
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