Specifications
- HOST SPECIES: Goat
- HOMOLOGY: Expected Species Reactivity based on sequence homology: Human, Mouse, Rat, Dog, Cow, Pig
- IMMUNOGEN: The immunogen for this antibody is: C-DFAKSRSQFH
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA
- APPLICATION NOTE: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 100kDa band in Human , Mouse and Rat Heart lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 193kDa according to NP_006166.3. The 100kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
- SPECIFICITY: This antibody is expected to recognize both reported isoforms (NP_006166.3; NP_932326.2).
Properties
- PURIFICATION: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
- CONCENTRATION: 500 ug/mL
- STORAGE CONDITIONS: Aliquot and store at -20°C. Minimize freezing and thawing.
Additional Info
- NCBI OFFICIAL SYMBOL: NRAP
- ADDITIONAL NAMES: NRAP, nebulin-related anchoring protein
- Protein Accession Number: NP_006166.3,NP_932326.2
- PROTEIN GI NUMBER: 46049092
- NCBI GENE ID NUMBER: 4892
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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