Specifications
- HOST SPECIES: Goat
- SPECIES REACTIVITY: Human, Mouse
- HOMOLOGY: Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
- IMMUNOGEN: The immunogen for this antibody is: DHFHNGHCEKGCNN
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, IF
- APPLICATION NOTE: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments gave an approx 27kDa band in all human, mouse and rat tissue and cell line lysates tested after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 210kDa according to NP_004548.3. The 27kDa band was successfully blocked by incubation with the immunizing peptide.Immunofluorescence: Strong expression of the protein seen in the nuclei and membranes of HeLa cells, and in the membranes of NIH3T3 cells. Recommended concentration: 10ug/ml.
- SPECIFICITY: The immunizong peptide represents part of the extracellular domain.
Properties
- PURIFICATION: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
- CONCENTRATION: 500 ug/mL
- STORAGE CONDITIONS: Aliquot and store at -20°C. Minimize freezing and thawing.
Additional Info
- NCBI OFFICIAL SYMBOL: NOTCH4
- ADDITIONAL NAMES: NOTCH4, DAQB-143M3.4, FLJ16302, INT3, MGC74442, NOTCH3, Notch homolog 4, neurogenic locus notch homolog protein 4
- Protein Accession Number: NP_004548.3
- PROTEIN GI NUMBER: 55770876
- NCBI GENE ID NUMBER: 4855
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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