- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (77%), Mouse: (77%)
- IMMUNOGEN: Rabbit NOD2 polyclonal antibody was raised against a synthetic peptide corresponding to 14 amino acids at the carboxy terminus of human NOD2.
The immunogen is located within the last 50 amino acids of NOD2.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, ICC, IF, WB
- APPLICATION NOTE: NOD2 antibody can be used for detection of Noxa by Western blot at 2 to 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
- SPECIFICITY: NOD2 has no cross-reaction with NOD1.
- POSITIVE CONTROL 1: Cat. No. 1201 - HeLa Cell Lysate
- POSITIVE CONTROL 2: Cat. No. 17-001 - HeLa Cell Slide
- PREDICTED MOLECULAR WEIGHT: 114 kDa
- PURIFICATION: NOD2 Antibody is immunoaffinity chromatography purified IgG.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: NOD2 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: NOD2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- NCBI OFFICIAL SYMBOL: NOD2
- ADDITIONAL NAMES: NOD2 Antibody: CD, ACUG, BLAU, IBD1, NLRC2, NOD2B, CARD15, CLR16.3, PSORAS1, Nucleotide-binding oligomerization domain-containing protein 2, Caspase recruitment domain-containing protein 15
- Protein Accession Number: Q9HC29
- PROTEIN GI NUMBER: 20137973
- NCBI GENE ID NUMBER: 64127
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
- NOD2 Antibody: Apaf-1 and NOD1 are members of a new family, which are involved in the regulation of apoptosis and immune response. Each of them contains a caspase recruitment domain (CARD) and a nucleotide-binding oligomerization domain (NOD). A third member in this family was recently identified and designated NOD2. NOD2 interacts with RICK via a homophilic CARD-CARD interaction. NOD2 activates NF-κB, which is regulated by its carboxy-terminal leucine-rich repeat domain that acts as an intracellular receptor for components of bacteria. The variants of NOD2, either a frameshift or a missense, were associated with Crohn's disease that is a main type of chronic inflammatory bowel disease.
- 1: Inohara N, Koseki T, del Peso L, et al. Nod1, an Apaf-1-like activator of caspase-9 and nuclear factor-κB. J. Biol. Chem. 1999; 274:14560-7.
- 2: Ogura Y, Inohara N, Benito A, et al. Nod2, a Nod1/Apaf-1 family member that is restricted to monocytes and activates NF-κB. J. Biol. Chem. 2001; 276:4812-8.
- 3: Hugot JP, Chamaillard M, et al. Association of NOD2 leucine-rich repeat variants with susceptibility to Crohn's disease. Nature 2001; 411:599-603.
- 4: Ogura Y, Bonen DK, Inohara N, et al. A frameshift mutation in NOD2 associated with susceptibility to Crohn's disease. Nature 2001; 411:603-6.
- FOR RESEARCH USE ONLY
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- Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
CATALOG NUMBER: 2513
- Size: 0.02 mg | 0.1 mg
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