- Trial Size
NKX2-2 Antibody
CATALOG NUMBER: 6743- Clonality: Polyclonal
- Tested Applications: E, IF, IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
Powered by BiozSpecifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: NKX2-2 antibody was raised against a 19 amino acid synthetic peptide near the center of human NKX2-2.
The immunogen is located within amino acids 90 - 140 of NKX2-2. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, IHC-P, WB
- APPLICATION NOTE: NKX2-2 antibody can be used for detection of NKX2-2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. - SPECIFICITY: NKX2-2 antibody is predicted to not cross-react with other NK2 homeobox family members.
- POSITIVE CONTROL 1: Cat. No. 1465 - Rat Kidney Tissue Lysate
- POSITIVE CONTROL 2: Cat. No. 10-401 - Human Kidney Tissue Slide
Properties
- PURIFICATION: NKX2-2 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: NKX2-2 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: NKX2-2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: NKX2-2
- ADDITIONAL NAMES: NKX2-2 Antibody: NKX2B, NKX2.2, NKX2B, Homeobox protein NK-2 homolog B
- Protein Accession Number: NP_002500
- PROTEIN GI NUMBER: 4505401
- NCBI GENE ID NUMBER: 4821
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- NKX2-2 Antibody: NKX2-2 (NK2 homeobox 2) is a member of a family of transcription factors that are involved in embryonic development and cell fate. NKX2-2 is crucial in the development of pancreatic islet cell differentiation and activates the ghrelin-promoter in these cells. NKX2-2 is expressed at the onset of pancreatic epithelium formation and becomes restricted to mature α-, β- and PP cells, and in NKX2-2-null mice embryos, all β-cells and most a-cells are replaced by ghrelin-positive cells. NKX2-2 is also thought to be involved in ventral neuronal patterning and floor plate development and commissural axon guidance.
- 1: Sussel L, Kalamaras J, Hartigan-O'Connor DJ, et al. Mice lacking the homeodomain transcription factor Nkx2.2 have diabetes due to arrested differentiation of pancreatic beta cells. Development 1998; 125:2213-21
- 2: Prado CL, Pugh-Bernard AE, Elghazi L, et al. Ghrelin cells replace insulin-producing beta cells in two mouse models of pancreas development. Proc. Natl. Acad. Sci. 2004; 101:2924-9.
- 3: Hill JT, Chao CS, Anderson KR, et al. Nkx2.2 activates the ghrelin promoter in pancreatic islet cells. Mol. Endocrinol. 2010; 381-90.
- 4: Briscoe J, Sussel L, Serup P, et al. Homeobox gene Nkx2.2 and specification of neuronal identity by graded Sonic hedgehog signaling. Nature 1999; 398:622-7.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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