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NIK AntibodyNIK Antibody
NIK Antibody
NIK Antibody
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NIK Antibody

CATALOG NUMBER: 1129
  • Clonality: Polyclonal
  • Tested Applications: E, ICC, IF, WB
  • Host Species: Rabbit
  • Species Reactivity: Human
  • Conjugate: Unconjugated
  • Size: 0.02 mg | 0.1 mg

Specifications

  • HOST SPECIES: Rabbit
  • SPECIES REACTIVITY: Human
  • HOMOLOGY: Predicted species reactivity based on immunogen sequence: Mouse: (94%)
  • IMMUNOGEN: NIK antibody was raised against a 17 amino acid peptide near the carboxy terminus of human NIK.
    The immunogen is located within the last 50 amino acids of NIK.
  • CONJUGATE: Unconjugated
  • TESTED APPLICATIONS: E, ICC, IF, WB
  • APPLICATION NOTE: NIK antibody can be used for detection of NIK by Western blot at 1 - 2 μg/mL.
    Antibody validated: Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
  • POSITIVE CONTROL 1: Cat. No. 1210 - HEK293 Cell Lysate
  • PREDICTED MOLECULAR WEIGHT: Predicted: 104 kDa

Properties

  • PURIFICATION: NIK Antibody is affinity chromatography purified via peptide column.
  • CLONALITY: Polyclonal
  • ISOTYPE: IgG
  • PHYSICAL STATE: Liquid
  • BUFFER: NIK Antibody is supplied in PBS containing 0.02% sodium azide.
  • CONCENTRATION: 1 mg/ml
  • STORAGE CONDITIONS: NIK antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

  • NCBI OFFICIAL SYMBOL: MAP3K14
  • ADDITIONAL NAMES: NIK Antibody: HS, NIK, HSNIK, FTDCR1B, Mitogen-activated protein kinase kinase kinase 14, NF-kappa-beta-inducing kinase, HsNIK
  • Protein Accession Number: Q99558
  • PROTEIN GI NUMBER: 92090612
  • NCBI GENE ID NUMBER: 9020
  • USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background

  • NIK Antibody: Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα, LPS and mitogens. A serine/threonine protein kinase which mediates NF-κB activation by IL-1, TNFα and CD95 was identified recently and designated NIK (for NF-κB inducing kinase). NIK is an activator of IκB kinase alpha and beta (IKKα and IKKβ). Therefore, NIK is a key molecule in the NF-κB signaling pathway leading to the induction of a variety of gene expression in response to proinflammatory cytokines and bacteria products.
  • 1: Malinin NL, Boldin MP, Kovalenko AV, et al. MAP3K-related kinase involved in NF-κB induction by TNF, CD95 and IL-1. Nature 1997; 385:540-4.
  • 2: Regnier CH, Song HY, Gao X, et al. Identification and characterization of an IκB kinase. Cell 1997; 90:373-83.
  • 3: Woronicz JD, Gao X, Cao Z, et al. IκB kinase-β: NF-κB activation and complex formation with IκB kinase-α and NIK. Science 1997; 278:866-9.
  • 4: Ling L, Cao Z, and Goeddel D. NF-κB-inducing kinase activates IKK-α by phosphorylation of Ser-176. Proc. Natl. Acad. Sci. USA 1998; 95:3792-7.

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER: 1129

  • Size: 0.02 mg | 0.1 mg
  • List Price: $89.00 – $395.00
Datasheet
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