- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Chicken: (85%)
- IMMUNOGEN: NIBRIN antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human NIBRIN.
The immunogen is located within amino acids 50 - 100 of NIBRIN.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, IHC-P, WB
- APPLICATION NOTE: NIBRIN antibody can be used for detection of NIBRIN by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
- SPECIFICITY: At least three alternatively spliced transcript isoforms of NIBRIN are known to exist.
- POSITIVE CONTROL 1: Cat. No. 1462 - Rat Lung Tissue Lysate
- POSITIVE CONTROL 2: Cat. No. 10-101 - Human Lung Tissue Slide
- PURIFICATION: NIBRIN Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: NIBRIN Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: NIBRIN antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- NCBI OFFICIAL SYMBOL: NBN
- ADDITIONAL NAMES: NIBRIN Antibody: ATV, NBS, P95, NBS1, AT-V1, AT-V2, Nibrin, Cell cycle regulatory protein p95
- Protein Accession Number: NP_002476
- PROTEIN GI NUMBER: 33356172
- NCBI GENE ID NUMBER: 4683
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
- NIBRIN Antibody: NIBRIN (NBN) is a member of the double-strand break repair complex MRE11/RAD50/NBN (MRN) which is involved in DNA double-strand break repair, DNA damage-induced checkpoint activation and plays a critical role in the maintenance of chromosome integrity. NIBRIN contains two modules found in cell cycle checkpoint proteins, a forkhead-associated domain adjacent to a breast cancer carboxy-terminal domain. Mutations in this gene are associated with Nijmegen breakage syndrome and maybe the cause of cancer predisposition and aplastic anemia.
- 1: Carney JP, Maser RS, Olivares H, et al. The hMre11/hRad50 protein complex and Nijmegen breakage syndrome: linkage of double-strand break repair to the cellular DNA damage response. Cell 1998; 93:477-86
- 2: Marcelain K, De La Torre C, Gonzalez P, et al. Roles of nibrin and AtM/ATR kinases on the G2 checkpoint under endogenous or radio-induced DNA damage. Biol. Res. 2005; 38:179-85.
- 3: Varon R, Vissinga C, Platzer M, et al. Nibrin, a novel DNA double-strand break repair protein, is mutated in Nijmegen breakage syndrome. Cell 1998; 93:467-76.
- 4: Heikkinen K, Karppinen SM, Soini Y. et al. Mutation screening of Mre11 complex genes: indication of RAD50 involvement in breast and ovarian cancer susceptibility. J. Med. Genet. 2003; 40:E131.
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page.
- Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
CATALOG NUMBER: 6841
- Size: 0.02 mg | 0.1 mg
- List Price: $89.00 – $395.00
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