- Trial Size
Nhe-1 Antibody
CATALOG NUMBER: 4377- Clonality: Polyclonal
- Tested Applications: ELISA, IF, IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rabbit: (95%), Pig: (95%), Bovine: (95%)
- IMMUNOGEN: Nhe-1 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of the human Nhe-1.
The immunogen is located within the last 50 amino acids of Nhe-1. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
- APPLICATION NOTE: Nhe-1 antibody can be used for detection of Nhe-1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. - SPECIFICITY: At least three isoforms of Nhe-1 are known to exist.
- POSITIVE CONTROL 1: Cat. No. 1465 - Rat Kidney Tissue Lysate
- POSITIVE CONTROL 2: Cat. No. 10-301 - Human Brain Tissue Slide
- PREDICTED MOLECULAR WEIGHT: Predicted: 52, 90 kDa
Observed: 50, 90 kDa
Properties
- PURIFICATION: Nhe-1 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: Nhe-1 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: Nhe-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: SLC9A1
- ADDITIONAL NAMES: Nhe-1 Antibody: APNH, NHE1, NHE-1, APNH1, APNH
- Protein Accession Number: P19634
- PROTEIN GI NUMBER: 127809
- NCBI GENE ID NUMBER: 6548
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Nhe-1 Antibody: The Na+/H+ antiporter (Nhe-1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells and is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, and tumor promoters. Nhe-1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. This occurs through a previously unrecognized structural link between Nhe-1 and the actin-binding proteins ezrin, radixin, and moesin, collectively referred to as ERM proteins. A structural role for Nhe-1 has been proposed in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. It is also thought that Nhe-1 play a role in hypertension.
- 1: Mendoza SA. The Na+-H+ antiport is a mediator of cell proliferation. Acta Paediatr. Scand. 1987; 76:545-7.
- 2: Denker SP, Huang DC, Orlowski J, et al. Direct binding of the NA—H exchanger NHE1 to ERM proteins regulates the cortical cytoskeleton and cell shape independently of H(+) translocation. Mol. Cell. 2000; 6:1425-36.
- 3: Cingolani HE, Rebolledo OR, Portiansky EL, et al. Regression of hypertensive myocardial fibrosis by NA (+)/H(+) exchange inhibition. Hypertension 2003; 41:373-7.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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