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NF- kappaB p65 (phospho Ser281) Antibody
NF- kappaB p65 (phospho Ser281) Antibody

NF- kappaB p65 (phospho Ser281) Antibody

CATALOG NUMBER: 80-048
  • Clonality: Polyclonal
  • Tested Applications: WB
  • Host Species: Rabbit
  • Species Reactivity: Human, Mouse, Rat
  • Conjugate: Unconjugated
  • Size: 0.1 mg

Specifications

  • HOST SPECIES: Rabbit
  • SPECIES REACTIVITY: Human, Mouse, Rat
  • IMMUNOGEN: NF- kappaB p65 (phospho Ser281) antibody was raised against a peptide sequence around phosphorylation site of serine281 (E-L-S (p)-E-P) derived from Human NF- kappaB .
  • CONJUGATE: Unconjugated
  • TESTED APPLICATIONS: WB
  • APPLICATION NOTE: Western Blot: 1:500~1:1000
  • SPECIFICITY: The antibody detects endogenous level of NF- kappaB p65 only when phosphorylated at serine 281.
  • PREDICTED MOLECULAR WEIGHT: 65 kDa

Properties

  • PURIFICATION: Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • CLONALITY: Polyclonal
  • ISOTYPE: IgG
  • PHYSICAL STATE: Liquid
  • BUFFER: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • CONCENTRATION: 1 mg/mL
  • STORAGE CONDITIONS: Store antibody at -20°C for up to one year.

Additional Info

  • NCBI OFFICIAL SYMBOL: RELA
  • ADDITIONAL NAMES: NFKB3, Nuclear factor NF-kappa-B p65 subunit, RELA, TF65, p65
  • Protein Accession Number: NP_001138610.1
  • PROTEIN GI NUMBER: 223468681
  • NCBI GENE ID NUMBER: 5970

Background

  • NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex.
  • 1: Hochrainer K, Racchumi G, Anrather J (2013)J Biol Chem 288, 285-93. Dai Y, et al. (2008) Clin Cancer Res 14, 549-58. Seldon MP, et al. (2007)J Immunol 179, 7840-51.

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER: 80-048

  • Size: 0.1 mg
  • List Price: $335.00
Datasheet
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