Specifications
- HOST SPECIES: Goat
- SPECIES REACTIVITY: Human
- HOMOLOGY: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
- IMMUNOGEN: The immunogen for this antibody is: DPCQADTIRNDITKT
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, IHC
- APPLICATION NOTE: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave an approx 80kDa band in Human Brain (cortex and hippocampus) lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 94.7kDa for the mature form (aa22-866) according to NP_001138579.1 and 88.8kDa (aa22-816) according to NP_001138580.1. The 80kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Prostate. Recommended concentration: 5ug/ml.
- SPECIFICITY: This antibody is expected to recognize all reported isoforms (NP_001138579.1; NP_006150.1; NP_001138581.1; NP_001138582.1). Reported variants represent identical protein: NP_001138580.1, NP_006150.1.
Properties
- PURIFICATION: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
- CONCENTRATION: 500 ug/mL
- STORAGE CONDITIONS: Aliquot and store at -20°C. Minimize freezing and thawing.
Additional Info
- NCBI OFFICIAL SYMBOL: NELL2
- ADDITIONAL NAMES: NELL2, NEL-like 2 (chicken), NRP2, NEL-like protein 2, NEL-related protein 2, neural epidermal growth factor-like 2, protein kinase C-binding protein NELL2
- Protein Accession Number: NP_001138579.1,NP_006150.1,NP_001138581.1,NP_001138582.1
- PROTEIN GI NUMBER: 5453766
- NCBI GENE ID NUMBER: 4753
Background
- 1: Kahle JJ, Gulbahce N, Shaw CA, Lim J, Hill DE, Barabási AL, Zoghbi HY.Comparison of an expanded ataxia interactome with patient medical records reveals a relationship between macular degeneration and ataxia.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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