NCL Antibody
CATALOG NUMBER: 57-876- Clonality: Polyclonal
- Tested Applications: IF, WB
- Host Species: Rabbit
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 400 ul
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Monkey
- IMMUNOGEN: This NCL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-304 amino acids from the Central region of human NCL.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: IF, WB
- APPLICATION NOTE: For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50 - PREDICTED MOLECULAR WEIGHT: 77 kDa
Properties
- PURIFICATION: This antibody is purified through a protein A column, followed by peptide affinity purification.
- CLONALITY: Polyclonal
- ISOTYPE: Rabbit Ig
- PHYSICAL STATE: Liquid
- BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: NCL
- ADDITIONAL NAMES: Nucleolin, Protein C23, NCL
- Protein Accession Number: P19338
- PROTEIN GI NUMBER: 90110781
- NCBI GENE ID NUMBER: 4691
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, isinvolved in the synthesis and maturation of ribosomes. It islocated mainly in dense fibrillar regions of the nucleolus. HumanNCL gene consists of 14 exons with 13 introns and spansapproximately 11kb. The intron 11 of the NCL gene encodes a smallnucleolar RNA, termed U20.
- 1: Ishimaru, D., et al. J. Biol. Chem. 285(35):27182-27191(2010)
- 2: Tulchin, N., et al. Am. J. Pathol. 176(3):1203-1214(2010)
- 3: Strang, B.L., et al. J. Virol. 84(4):1771-1784(2010)
- 4: Bertrand, L., et al. J. Virol. 84(1):109-118(2010)
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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$35 Dry Ice Shipment fee may be required on some items.
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