Specifications
- HOST SPECIES: Mouse
- SPECIES REACTIVITY: Human
- IMMUNOGEN: The monoclonal antibody was produced in BALB/c mice using recombinant human NAP-2 as the immunizing antigen.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, WB
- APPLICATION NOTE: ELISA:
In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human NAP-2 when used with our biotinylated antigen affinity purified anti-human NAP-2 (XP-5244Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
Western blot:
To detect hNAP-2 by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNAP-2 is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.
Properties
- PURIFICATION: This IgG1/K antibody was purified from a Protein A chromatography column.
- CLONALITY: Monoclonal
- PHYSICAL STATE: Lyophilized
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: NAP-2 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.
Additional Info
- NCBI OFFICIAL SYMBOL: PPBP
- ADDITIONAL NAMES: PPBP, CXCL7, CTAP3, SCYB7, TGB1, THBGB1, Platelet basic protein, C-X-C motif chemokine 7, PBP, TC1, TC2, TGB, LDGF, MDGF, B-TG1, NAP-2, THBGB, LA-PF4, Beta-TG, CTAPIII, CTAP-III
- Protein Accession Number: P02775
- PROTEIN GI NUMBER: 129874
- NCBI GENE ID NUMBER: 5473
- USER NOTE: Centrifuge vial prior to opening.
Background
- NAP2 is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and sythesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: This product is for research use only.
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