NAP-2 Antibody
CATALOG NUMBER: XP-5245- Clonality: Polyclonal
- Tested Applications: E, WB
- Host Species: Goat
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 0.1 mg
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- HOST SPECIES: Goat
- SPECIES REACTIVITY: Human
- IMMUNOGEN: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hNAP-2 (human Neutrophil Activating Protein-2).
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, WB
- APPLICATION NOTE: ELISA:
To detect hNAP-2 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hNAP-2.
Sandwich:
To detect hNAP-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human NAP-2 (XP-5245Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNAP-2.
Western Blot:
To detect hNAP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNAP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Properties
- PURIFICATION: Anti-hNAP-2 specific antibody was purified by affinity chromatography employing immobilized hNAP-2 matrix.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Lyophilized
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: NAP-2 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: PPBP
- ADDITIONAL NAMES: PBP, TC1, TC2, TGB, LDGF, MDGF, TGB1, B-TG1, CTAP3, CXCL7, NAP-2, SCYB7, THBGB, LA-PF4, THBGB1, Beta-TG, CTAPIII, CTAP-III, Platelet basic protein, C-X-C motif chemokine 7, PBP
- Protein Accession Number: P02775
- PROTEIN GI NUMBER: 129874
- NCBI GENE ID NUMBER: 5473
- USER NOTE: Centrifuge vial prior to opening.
Background
- NAP2 is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and sythesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: This product is for research use only.
Shipping Info
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