- Trial Size
NALP7 Antibody
CATALOG NUMBER: 5963- Clonality: Polyclonal
- Tested Applications: ELISA, IF, IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- IMMUNOGEN: NALP7 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human NALP7.
The immunogen is located within amino acids 90 - 140 of NALP7. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
- APPLICATION NOTE: NALP7 antibody can be used for detection of NALP7 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. - POSITIVE CONTROL 1: Cat. No. 1204 - K562 Cell Lysate
- POSITIVE CONTROL 2: Cat. No. 10-301 - Human Brain Tissue Slide
Properties
- PURIFICATION: NALP7 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: NALP7 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: NALP7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: NLRP7
- ADDITIONAL NAMES: NALP7 Antibody: HYDM, PAN7, NALP7, NOD12, PYPAF3, CLR19.4, NACHT, LRR and PYD domains-containing protein 7, Nucleotide-binding oligomerization domain protein 12
- Protein Accession Number: NP_631915
- PROTEIN GI NUMBER: 46049100
- NCBI GENE ID NUMBER: 199713
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- NALP7 Antibody: NOD proteins include the apoptosis regulator APAF1 (apoptotic protease activating factor 1) and mammalian NOD-LRR proteins. NALP7, also known as PYPAF3, a member of the related PYRIN-containing APAF1-like proteins (PYPAFs/NALPs) is thought to play a crucial role in cell proliferation. NALP7 has a C-terminal leucine-rich repeat (LRR) region, an N-terminal Pyrin domain (PYD) followed by a NACHT domain, and a NACHT-associated domain. It is expressed in numerous tissues including uterus and ovary, with low levels in heart and brain. NALP7 inhibits caspase-1-dependent interleukin-1β secretion and is a feedback regulator of interleukin-1β secretion. Defects in the NALP7 gene are known to cause the formation of a hydatidiform mole (HYDM) and reduce the growth of carcinoma cell lines.
- 1: Tschopp J, Martinon F, and Burns K. NALPs: a novel protein family involved in inflammation. Nat. Rev. Mol. Cell Biol.2003; 4:95-104.
- 2: Kinoshita T, Wang Y, Hasegawa M, et al. PYPAF3, a PYRIN-containing APAF-1-like protein, is a feedback regulator of caspase-1-dependent interleukin-1beta secretion. J. Biol. Chem.2005; 280:21720-5.
- 3: Okada K, Hirota E, Mizutani Y, et al. Oncogenic role of NALP7 in testicular seminomas. Cancer Sci.2004; 95: 949-54.
- 4: Murdoch S, Djuric U, Mazhar B, et al. Mutations in NALP7 cause recurrent hydatidiform moles and reproductive wastage in humans. Nat. Genet.2006; 38: 300-2.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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