- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- IMMUNOGEN: This MYO1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-304 amino acids from the Central region of human MYO1A.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: Flow, WB
- APPLICATION NOTE: For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
- PREDICTED MOLECULAR WEIGHT: 118 kDa
- PURIFICATION: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
- CLONALITY: Polyclonal
- ISOTYPE: Rabbit Ig
- PHYSICAL STATE: Liquid
- BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- NCBI OFFICIAL SYMBOL: MYO1A
- ADDITIONAL NAMES: Unconventional myosin-Ia, Brush border myosin I, BBM-I, BBMI, Myosin I heavy chain, MIHC, MYO1A, MYHL
- Protein Accession Number: Q9UBC5
- PROTEIN GI NUMBER: 13431715
- NCBI GENE ID NUMBER: 4640
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
- MYO1A belongs to the myosin superfamily. Myosins are molecular motors that, upon interaction with actin filaments, utilize energy from ATP hydrolysis to generate mechanical force. Each myosin has a conserved N-terminal motor domain that contains both ATP-binding and actin-binding sequences. Following the motor domain is a light-chain-binding 'neck' region containing 1-6 copies of a repeat element, the IQ motif, that serves as a binding site for calmodulin or other members of the EF-hand superfamily of calcium-binding proteins. At the C-terminus, each myosin class has a distinct tail domain that serves in dimerization, membrane binding, protein binding, and/or enzymatic activities and targets each myosin to its particular subcellular location. The kidney epithelial cell line, LLC-PK1-CL4 (CL4), forms a well ordered brush border (BB) on its apical surface. Experiments indicate that the brush border population of the protein turns over rapidly, while its head and tail domains interact transiently with the core actin and plasma membrane, respectively. A rapidly exchanging pool of the protein envelops an actin core bundle that, by comparison, is static in structure.
- 1: D'Adamo,P., Pinna,M. Hum. Genet. 112 (3), 319-320 (2003)
- 2: Hoshimaru,M., Fujio,Y. J. Biochem. 106 (3), 455-459 (1989)
- 3: Durrbach,A., Collins,K. Proc. Natl. Acad. Sci. U.S.A. 93 (14), 7053-7058 (1996)
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page.
- Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
CATALOG NUMBER: 62-841
- Size: 400 ul
- List Price: $445.00
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