MRPS15 Antibody
CATALOG NUMBER: 58-526- Clonality: Polyclonal
- Tested Applications: WB
- Host Species: Rabbit
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 400 ul
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- IMMUNOGEN: This MRPS15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124-153 amino acids from the Central region of human MRPS15.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: WB
- APPLICATION NOTE: For WB starting dilution is: 1:1000
- PREDICTED MOLECULAR WEIGHT: 30 kDa
Properties
- PURIFICATION: This antibody is purified through a protein A column, followed by peptide affinity purification.
- CLONALITY: Polyclonal
- ISOTYPE: Rabbit Ig
- PHYSICAL STATE: Liquid
- BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: MRPS15
- ADDITIONAL NAMES: 28S ribosomal protein S15, mitochondrial, MRP-S15, S15mt, MRPS15, RPMS15
- Protein Accession Number: P82914
- PROTEIN GI NUMBER: 13633907
- NCBI GENE ID NUMBER: 64960
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Mammalian mitochondrial ribosomal proteins are encoded bynuclear genes and help in protein synthesis within themitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of asmall 28S subunit and a large 39S subunit. They have an estimated75% protein to rRNA composition compared to prokaryotic ribosomes,where this ratio is reversed. Another difference between mammalianmitoribosomes and prokaryotic ribosomes is that the latter containa 5S rRNA. Among different species, the proteins comprising themitoribosome differ greatly in sequence, and sometimes inbiochemical properties, which prevents easy recognition by sequencehomology. This gene encodes a 28S subunit protein that belongs tothe ribosomal protein S15P family. The encoded protein is more thantwo times the size of its E. coli counterpart, with the 12S rRNAbinding sites conserved. Between human and mouse, the encodedprotein is the least conserved among small subunit ribosomalproteins. Pseudogenes corresponding to this gene are found onchromosomes 15q and 19q.
- 1: Zhang, Z., et al. Genomics 81(5):468-480(2003)
- 2: Kenmochi, N., et al. Genomics 77 (1-2), 65-70 (2001) :
- 3: Suzuki, T., et al. J. Biol. Chem. 276(35):33181-33195(2001)
- 4: Cavdar Koc, E., et al. J. Biol. Chem. 276(22):19363-19374(2001)
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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