MRPL28 Antibody
CATALOG NUMBER: 19-930- Clonality: Polyclonal
- Tested Applications: IF, IHC, IP, WB
- Host Species: Rabbit
- Species Reactivity: Human, Rat
- Conjugate: Unconjugated
- Size: 50 uL
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Rat
- IMMUNOGEN: Recombinant fusion protein containing a sequence corresponding to amino acids 1-256 of human MRPL28 (NP_006419.2).
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: IF, IHC, IP, WB
- APPLICATION NOTE: WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
IP: 1:50 - 1:200 - POSITIVE CONTROL 1: MCF7
- POSITIVE CONTROL 2: HepG2
- POSITIVE CONTROL 3: 293T
- POSITIVE CONTROL 4: A-549
- PREDICTED MOLECULAR WEIGHT: Observed: 30kDa
Properties
- PURIFICATION: Affinity purification
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Store at -20°C. Avoid freeze / thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: MRPL28
- ADDITIONAL NAMES: 39S ribosomal protein L28, mitochondrial, L28mt, MRP-L28, Melanoma antigen p15, Melanoma-associated antigen recognized by T-lymphocytes, MRPL28, MAAT1
- NCBI GENE ID NUMBER: 10573
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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