Mouse Cdk8 Antibody
CATALOG NUMBER: 58-365- Clonality: Polyclonal
- Tested Applications: WB
- Host Species: Rabbit
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 400 ul
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Zebrafish
- IMMUNOGEN: This Mouse Cdk8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 245-272 amino acids from the Central region of mouse Cdk8.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: WB
- APPLICATION NOTE: For WB starting dilution is: 1:1000
- PREDICTED MOLECULAR WEIGHT: 53 kDa
Properties
- PURIFICATION: This antibody is purified through a protein A column, followed by peptide affinity purification.
- CLONALITY: Polyclonal
- ISOTYPE: Rabbit Ig
- PHYSICAL STATE: Liquid
- BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: Cdk8
- ADDITIONAL NAMES: Cyclin-dependent kinase 8, Cell division protein kinase 8, Mediator complex subunit CDK8, Mediator of RNA polymerase II transcription subunit CDK8, Cdk8
- Protein Accession Number: Q8R3L8
- NCBI GENE ID NUMBER: 264064
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation (By similarity).
- 1: Alarcon, C., et al. Cell 139(4):757-769(2009)
- 2: Westerling, T., et al. Mol. Cell. Biol. 27(17):6177-6182(2007)
- 3: Jang, M.K., et al. Mol. Cell 19(4):523-534(2005)
- 4: Tomomori-Sato, C., et al. J. Biol. Chem. 279(7):5846-5851(2004)
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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