- Trial Size
MMS21 Antibody
CATALOG NUMBER: 5759- Clonality: Polyclonal
- Tested Applications: ELISA, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (80%)
- IMMUNOGEN: MMS21 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human MMS21.
The immunogen is located within amino acids 170 - 220 of MMS21. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, WB
- APPLICATION NOTE: MMS21 antibody can be used for detection of MMS21 by Western blot at 0.5 - 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. - POSITIVE CONTROL 1: Cat. No. 1210 - HEK293 Cell Lysate
- PREDICTED MOLECULAR WEIGHT: Predicted: 27 kDa
Observed: 30 kDa
Properties
- PURIFICATION: MMS21 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: MMS21 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: MMS21 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: NSMCE2
- ADDITIONAL NAMES: MMS21 Antibody: NSE2, MMS21, ZMIZ7, C8orf36, E3 SUMO-protein ligase NSE2, MMS21 homolog, hMMS21
- Protein Accession Number: NP_775956
- PROTEIN GI NUMBER: 27734761
- NCBI GENE ID NUMBER: 286053
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- MMS21 Antibody: MMS21, also known as NSE2, is a SUMO ligase that in combination with the SMC5/6 complex is required for the prevention of DNA damage induced apoptosis by facilitating DNA repair in human cells. MMS21-dependent sumoylation is integral and important to the cohesion mechanism and mitotic progression; this function appears to be independent of SMC6. MMS21 mediates SUMO attachment to various proteins such as SMC6L1 and TRAX, and possibly the cohesin components RAD21 and STAG2.
- 1: Potts PR and Yu H. Human MMS21/NSE2 is a SUMO ligase required for DNA repair. Mol. Cell. Biol. 2005; 25:7021-32.
- 2: Behlke-Steinert S, Touat-Todeschini L, Skoufias DA, et al. SMC5 and MMS21 are required for chromosome cohesion and mitotic progression. Cell Cycle 2009; 8:2211-8.
- 3: Bermudez-Lopez M, Ceschia A, de Piccoli G, et al. The Smc5/6 complex is required for dissolution of DNA-mediated sister chromatid linkages. Nucleic Acids Res. 2010; 38:6502-12.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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