MIP-1a Antibody (biotin)
CATALOG NUMBER: XP-5232Bt- Clonality: Polyclonal
- Tested Applications: E, WB
- Host Species: Goat
- Species Reactivity: Human
- Conjugate: Biotin
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- HOST SPECIES: Goat
- SPECIES REACTIVITY: Human
- IMMUNOGEN: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hMIP-1-alpha (human MIP-1-alpha).
- CONJUGATE: Biotin
- TESTED APPLICATIONS: E, WB
- APPLICATION NOTE: ELISA:
Sandwich:
To detect hMIP-1α by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human MIP-1α (XP-5232) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-1α.
Western Blot:
To detect hMIP-1-α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-1-α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Properties
- PURIFICATION: Anti-hMIP-1-alpha specific antibody was purified by affinity chromatography and then biotinylated.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Lyophilized
- STORAGE CONDITIONS: MIP-1a antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: CCL3
- ADDITIONAL NAMES: MIP1A, SCYA3, G0S19-1, LD78ALPHA, MIP-1-alpha, MIP1A, C-C motif chemokine 3, Macrophage inflammatory protein 1-alpha
- Protein Accession Number: P10147
- PROTEIN GI NUMBER: 127078
- NCBI GENE ID NUMBER: 6348
- USER NOTE: Centrifuge vial prior to opening.
Background
- MIP1 alpha and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77% and 70% amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: This product is for research use only.
Shipping Info
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