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Home / Cancer / Melanoma Antibody [PNL2]
Melanoma Antibody [PNL2]Melanoma Antibody [PNL2]
Melanoma Antibody [PNL2]
Melanoma Antibody [PNL2]

Melanoma Antibody [PNL2]

CATALOG NUMBER: 33-528
  • Clonality: Monoclonal
  • Tested Applications: IF, IHC-P, WB
  • Host Species: Mouse
  • Species Reactivity: Dog, Human, Mouse
  • Conjugate: Unconjugated
  • Size: 100 ug

Specifications

  • HOST SPECIES: Mouse
  • SPECIES REACTIVITY: Dog, Human, Mouse
  • IMMUNOGEN: Melanocyte antigen was used as the immunogen for the Melanoma antibody PNL2.
  • CONJUGATE: Unconjugated
  • TESTED APPLICATIONS: IF, IHC-P, WB
  • APPLICATION NOTE: Immunofluorescence: 0.5-1 ug/ml
    Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)
    Prediluted format: incubate for 30 min at RT (2)
    Optimal dilution of the Melanoma antibody PNL2 should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Properties

  • PURIFICATION: Protein G affinity chromatography
  • CLONALITY: Monoclonal
  • ISOTYPE: IgG1
  • PHYSICAL STATE: Liquid
  • BUFFER: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
  • CONCENTRATION: 0.2 mg/mL
  • STORAGE CONDITIONS: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.

Additional Info

  • USER NOTE: Optimal dilutions for each application to be determined by the researcher

Background

  • Anti-PNL2 is a novel monoclonal antibody, which has recently been introduced as an immunohistochemical reagent to stain melanocytes and tumors derived therefrom. The antigen recognized by PNL2 is different from Melan A and gp100. Its epitope is not destroyed by digestion with neuraminidase i.e. its epitope id not glycosylated. Anti-PNL2 may be most useful because of its high sensitivity for metastatic melanoma (87%), as opposed to 76% for anti-HMB45 and 82% for anti-MART-1. Anti-PNL2 labels intra-epidermal nevi while the dermal component of compound nevi are largely non-reactive with anti-PNL2. Antibodies against PNL2, MART-1 (Melan A) and HMB45 stain most clear cell sarcoma cells and a few cells in angio-myolipomas and lymphangioleiomyomatosis. Anti-PNL2 is a useful antibody for the identification of melanomas and clear cell sarcomas. Differential diagnosis is aided by the results from a panel of antibodies, including antibodies against HMB45, MART-1, tyrosinase, and MiTF.

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER: 33-528

  • Size: 100 ug
  • List Price: $555.00
Datasheet
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