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Home / Dog / Melan-A Antibody [MLANA/788]
Melan-A Antibody [MLANA/788]Melan-A Antibody [MLANA/788]
Melan-A Antibody [MLANA/788]
Melan-A Antibody [MLANA/788]

Melan-A Antibody [MLANA/788]

CATALOG NUMBER: 33-360
  • Clonality: Monoclonal
  • Tested Applications: Flow, IF, IHC-P, WB
  • Host Species: Mouse
  • Species Reactivity: Dog, Human, Mouse, Rat
  • Conjugate: Unconjugated
  • Size: 100 ug

Specifications

  • HOST SPECIES: Mouse
  • SPECIES REACTIVITY: Dog, Human, Mouse, Rat
  • IMMUNOGEN: Recombinant human MLANA protein was used as the immunogen for the Melan-A antibody.
  • CONJUGATE: Unconjugated
  • TESTED APPLICATIONS: Flow, IF, IHC-P, WB
  • APPLICATION NOTE: Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
    Immunofluorescence: 0.5-1 ug/ml
    Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)
    Western blot: 0.5-1 ug/ml
    Prediluted format : incubate for 30 min at RT (2)
    Optimal dilution of the Melan-A antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Properties

  • PURIFICATION: Protein G affinity chromatography
  • CLONALITY: Monoclonal
  • ISOTYPE: IgG1
  • PHYSICAL STATE: Liquid
  • BUFFER: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
  • CONCENTRATION: 0.2 mg/mL
  • STORAGE CONDITIONS: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.

Additional Info

  • NCBI OFFICIAL SYMBOL: MLANA
  • ADDITIONAL NAMES: MLANA, Antigen LB39-AA, Antigen SK29-AA, Melan-A, Protein Melan-A, MART-1, MART1
  • NCBI GENE ID NUMBER: 2315
  • USER NOTE: Optimal dilutions for each application to be determined by the researcher

Background

  • This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This mAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER: 33-360

  • Size: 100 ug
  • List Price: $555.00
Datasheet
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