MDM2 Antibody
CATALOG NUMBER: 56-493- Clonality: Polyclonal
- Tested Applications: Flow, IF, IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 400 ul
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- IMMUNOGEN: This MDM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 141-176 amino acids from human MDM2.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: Flow, IF, IHC-P, WB
- APPLICATION NOTE: For FACS starting dilution is: 1:25
For WB starting dilution is: 1:2000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50 - PREDICTED MOLECULAR WEIGHT: 55 kDa
Properties
- PURIFICATION: This antibody is purified through a protein A column, followed by peptide affinity purification.
- CLONALITY: Polyclonal
- ISOTYPE: Rabbit Ig
- PHYSICAL STATE: Liquid
- BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: MDM2
- ADDITIONAL NAMES: E3 ubiquitin-protein ligase Mdm2, 632-, Double minute 2 protein, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2, MDM2
- Protein Accession Number: Q00987
- PROTEIN GI NUMBER: 266516
- NCBI GENE ID NUMBER: 4193
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- MDM2 is a target of the transcription factor tumor protein p53. This protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of MDM2 can result in excessive inactivation of tumor proteinp53, diminishing its tumor suppressor function. This protein has E3ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle,apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5.
- 1: Burch, L.R., et al., J. Mol. Biol. 337(1):115-128 (2004).
- 2: Schon, O., et al., J. Mol. Biol. 336(1):197-202 (2004).
- 3: Mantesso, A., et al., J. Oral Pathol. Med. 33(2):96-101 (2004).
- 4: Shmueli, A., et al., Mol. Cell 13(1):4-5 (2004).
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
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