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MD-2 Antibody [9F1B1]MD-2 Antibody [9F1B1]MD-2 Antibody [9F1B1]
MD-2 Antibody [9F1B1]
MD-2 Antibody [9F1B1]
MD-2 Antibody [9F1B1]
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MD-2 Antibody [9F1B1]

CATALOG NUMBER: PM-4845
  • Clonality: Monoclonal
  • Tested Applications: E, IF, IHC-P, WB
  • Host Species: Mouse
  • Species Reactivity: Human, Mouse
  • Conjugate: Unconjugated
  • Size: 0.02 mg | 0.1 mg

Specifications

  • HOST SPECIES: Mouse
  • SPECIES REACTIVITY: Human, Mouse
  • IMMUNOGEN: Mouse monoclonal MD-2 antibody was raised against a recombinant protein corresponding to amino acids 2 to 160 of human MD-2.
  • CONJUGATE: Unconjugated
  • TESTED APPLICATIONS: E, IF, IHC-P, WB
  • APPLICATION NOTE: MD-2 antibody can be used for detection of MD-2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
    Antibody validated: Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
  • POSITIVE CONTROL 1: Cat. No. 95-108 - MD-2 Recombinant Protein
  • POSITIVE CONTROL 2: Cat. No. 10-901 - Human Spleen Tissue Slide

Properties

  • PURIFICATION: MD-2 Monoclonal Antibody is immunoaffinity chromotography purified IgG.
  • CLONALITY: Monoclonal
  • ISOTYPE: IgG1
  • PHYSICAL STATE: Liquid
  • BUFFER: MD-2 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
  • CONCENTRATION: 1 mg/mL
  • STORAGE CONDITIONS: MD-2 monoclonal antibody can be stored at -20°C, stable for one year.

Additional Info

  • NCBI OFFICIAL SYMBOL: LY96
  • ADDITIONAL NAMES: MD-2 Antibody [9F1B1] : MD2, MD-2, ly-96, ESOP-1
  • Protein Accession Number: NP_056179
  • PROTEIN GI NUMBER: 223555998
  • NCBI GENE ID NUMBER: 23643
  • USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background

  • MD-2 Monoclonal Antibody: MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2; e.g., human MD-2 transfected into mouse cells can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.
  • 1: O'Neill LAJ, Fitzgerald FA, and Bowie AG. The Toll-IL-1 receptor adaptor family grows to five members. Trends in Imm. 2003; 24:286-9.
  • 2: Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77.
  • 3: Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
  • 4: Janeway CA Jr and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216.

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER: PM-4845

  • Size: 0.02 mg | 0.1 mg
  • List Price: $89.00 – $459.00
Datasheet
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