MCP-3 Antibody [PEPY042_hMCP3]
CATALOG NUMBER: XP-5733-M- Clonality: Monoclonal
- Tested Applications: ELISA, Neutralization Assay, WB
- Host Species: Mouse
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 0.5 mg
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- HOST SPECIES: Mouse
- SPECIES REACTIVITY: Human
- IMMUNOGEN: The monoclonal antibody was produced in C3H/HeJ mice using recombinant hMCP-3 as the immunizing antigen.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, Neutralization Assay, WB
- APPLICATION NOTE: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hMCP-3 (100ng/mL), a concentration of 1 - 3 μg/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect recombinant human MCP-3 when used with our biotinylated antigen affinity purified anti-human MCP-3 (XP-5221Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
Western Blot:
To detect hMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-3 is 2.0-4.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.
Properties
- PURIFICATION: This IgG1 antibody was purified from ascites fluid on a Protein A affinity column.
- CLONALITY: Monoclonal
- PHYSICAL STATE: Lyophilized
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: MCP-3 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.
Additional Info
- NCBI OFFICIAL SYMBOL: CCL7
- ADDITIONAL NAMES: Monocyte chemotactic protein 3, MCP3, SCYA6, SCYA7, C-C motif chemokine 7, Monocyte chemoattractant protein 3, MCP-3, FIC, MARC, NC28
- Protein Accession Number: P80098
- PROTEIN GI NUMBER: 1170890
- NCBI GENE ID NUMBER: 6354
- USER NOTE: Centrifuge vial prior to opening.
Background
- MCP-3 is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: This product is for research use only.
Shipping Info
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