MAX Antibody
CATALOG NUMBER: 45-088- Clonality: Polyclonal
- Tested Applications: ELISA, IF, IHC, WB
- Host Species: Goat
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 0.1 mg
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- HOST SPECIES: Goat
- SPECIES REACTIVITY: Human
- HOMOLOGY: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
- IMMUNOGEN: The immunogen for this antibody is: C-EEPQSRKKLRMEAS
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, IF, IHC, WB
- APPLICATION NOTE: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 22kDa band observed in lysates of cell line Jurkat (calculated MW of 18.3kDa according to NP_002373.3). Recommended concentration: 0.01-0.03ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Prostate. Recommended concentration: 10ug/ml.Immunofluorescence: Expression of the protein seen in the cytoplasm and nuclei of A431 and U251 cells. Recommended concentration: 10ug/ml.
Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
- SPECIFICITY: This antibody is expected to recognize both reported isoforms (NP_002373.3; NP_660087.1).
- POSITIVE CONTROL 1: Cat. No. 1205 - Jurkat Cell Lysate
Properties
- PURIFICATION: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
- CONCENTRATION: 500 ug/mL
- STORAGE CONDITIONS: Aliquot and store at -20°C. Minimize freezing and thawing.
Additional Info
- NCBI OFFICIAL SYMBOL: MAX
- ADDITIONAL NAMES: MAX, MAX protein, MGC10775, MGC11225, MGC18164, MGC34679, MGC36767, myc-associated factor X, helix-loop-helix zipper protein, MYC associated factor X, bHLHd4, bHLHd5, bHLHd6, bHLHd7, bHLHd8, orf1
- Protein Accession Number: NP_002373.3,NP_660087.1
- PROTEIN GI NUMBER: 21704261
- NCBI GENE ID NUMBER: 4149
Background
- 1: Blackwood EM, Eisenman RN. Max: a helix-loop-helix zipper protein that forms a sequence-specific DNA-binding complex with Myc. Science 1991 Mar 8;251(4998):1211-7
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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