- Trial Size
MAP1LC3B Antibody
CATALOG NUMBER: 7581- Clonality: Polyclonal
- Tested Applications: E, IF, IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (100%)
- IMMUNOGEN: MAP1LC3B antibody was raised against an 18 amino acid peptide near the amino terminus of human MAP1LC3B.
The immunogen is located within the first 50 amino acids of MAP1LC3B. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, IHC-P, WB
- APPLICATION NOTE: MAP1LC3B antibody can be used for detection of MAP1LC3B by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. - SPECIFICITY: MAP1LC3B antibody is human, mouse and rat reactive. Multiple isoforms MAP1LC3B are known to exist. MAP1LC3B antibody is predicted to not cross-react with MAP1LC3A or MAP1LC3C
- POSITIVE CONTROL 1: Cat. No. 1303 - Human Brain Tissue Lysate
- POSITIVE CONTROL 2: Cat. No. 10-301 - Human Brain Tissue Slide
- PREDICTED MOLECULAR WEIGHT: Predicted: 14 kDa
Observed: 14 kDa
Properties
- PURIFICATION: MAP1LC3B antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: MAP1LC3B antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: MAP1LC3B antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Additional Info
- NCBI OFFICIAL SYMBOL: MAP1LC3B
- ADDITIONAL NAMES: MAP1LC3B Antibody: LC3B, ATG8F, MAP1LC3B-a, MAP1A/1BLC3, MAP1ALC3, Autophagy-related protein LC3 B
- Protein Accession Number: NP_073729
- PROTEIN GI NUMBER: 12383056
- NCBI GENE ID NUMBER: 81631
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and plasticity (1). MAP1LC3B belongs to the MAP1 LC3 family and it includes 3 different light chains, LC1, LC2 and LC3 (2). MAP1LC3B is involved in formation of autophagosomal vacuoles (autophagosomes) (3). It is most abundant in heart, brain, skeletal muscle and testis. MAP1LC3B is essential for autophagy and associated to the autophagosome membranes after processing (4).
- 1: Mandelkow E and Mandelkow EM. Microtubules and microtubule-associated proteins. Curr. Opin. Cell Biol. 1995; 7:72-81.
- 2: Fink JK, Jones SM, Esposito C, et al. Human microtubule-associated protein 1A (MAP1A) gene: genomic organization, cDNA sequence, and developmental and tissue-specific expression. Genomics 1996; 35:577-85.
- 3: Colecchia D, Strambi A, Sanzone S, et al. MAPK15/ERK8 stimulates autophagy by interacting with LC3 and GABARAP proteins. Autophagy 2012; 8:1724-40.
- 4: Kabeya Y, Mizushima N, Ueno T, et al. LC3, a mammalian homolog of yeast Apg8p, is localized in autophagosome membrane after processing. EMBO J. 2000; 19:5720-8.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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