Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine, Rat
- IMMUNOGEN: This MAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90-117 amino acids from the Central region of human MAF1.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: Flow, IHC-P, WB
- APPLICATION NOTE: For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50 - PREDICTED MOLECULAR WEIGHT: 29 kDa
Properties
- PURIFICATION: This antibody is purified through a protein A column, followed by peptide affinity purification.
- CLONALITY: Polyclonal
- ISOTYPE: Rabbit Ig
- PHYSICAL STATE: Liquid
- BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: MAF1
- ADDITIONAL NAMES: Repressor of RNA polymerase III transcription MAF1 homolog, MAF1
- Protein Accession Number: Q9H063
- PROTEIN GI NUMBER: 296434582
- NCBI GENE ID NUMBER: 84232
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- This gene encodes a protein that is similar to Maf1, aSaccharomyces cerevisiae protein highly conserved in eukaryoticcells. Yeast Maf1 is a negative effector of RNA polymerase III (PolIII). It responds to changes in the cellular environment andrepresses pol III transcription. Biochemical studies identified theinitiation factor TFIIIB as a target for Maf1-dependent repression.
- 1: Kantidakis, T., et al. Proc. Natl. Acad. Sci. U.S.A. 107(26):11823-11828(2010)
- 2: Shor, B., et al. J. Biol. Chem. 285(20):15380-15392(2010)
- 3: Johnson, S.S., et al. Mol. Cell 26(3):367-379(2007)
- 4: Lamesch, P., et al. Genomics 89(3):307-315(2007)
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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