M-CSF Antibody
CATALOG NUMBER: XP-5228- Clonality: Polyclonal
- Tested Applications: ELISA, Neutralization Assay, WB
- Host Species: Goat
- Species Reactivity: Mouse
- Conjugate: Unconjugated
- Size: 0.1 mg
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- HOST SPECIES: Goat
- SPECIES REACTIVITY: Mouse
- IMMUNOGEN: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mM-CSF (Murine M-CSF).
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, Neutralization Assay, WB
- APPLICATION NOTE: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mM-CSF (1.5 ng/mL), a concentration of 0.05 - 0.08 μg/mL of this antibody is required.
ELISA:
To detect mM-CSF by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mM-CSF.
Sandwich:
To detect mM-CSF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine M-CSF (XP-5228Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mM-CSF.
Western Blot:
To detect mM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Properties
- PURIFICATION: Anti-mM-CSF specific antibody was purified by affinity chromatography employing immobilized mM-CSF matrix.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Lyophilized
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: M-CSF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: Csf1
- ADDITIONAL NAMES: op, Csfm, MCSF, C87615, Macrophage colony-stimulating factor 1CSF-1
- Protein Accession Number: P07141
- PROTEIN GI NUMBER: 1345843
- NCBI GENE ID NUMBER: 12977
- USER NOTE: Centrifuge vial prior to opening.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: This product is for research use only.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
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