Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: Anti-LSD1 antibody (3761) was raised against a peptide corresponding to 17 amino acids near the amino terminus of human LSD1.
The immunogen is located within amino acids 40-90 of LSD1. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, IHC-P, WB
- APPLICATION NOTE: WB: 0.2 - 2 μg/mL; IHC-P: 2 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry and Immunofluorescence in human samples. All other applications and species not yet tested. - POSITIVE CONTROL 1: Cat. No. 1286 - P815 Cell Lysate
- POSITIVE CONTROL 2: Cat. No. 1224 - Daudi Cell Lysate
- POSITIVE CONTROL 3: Cat. No. 1205 - Jurkat Cell Lysate
- POSITIVE CONTROL 4: Cat. No. 1211 – HepG2 Cell Lysate
- PREDICTED MOLECULAR WEIGHT: Predicted: 93kD
Observed: 110 kD
Advanced Validation
- VALIDATION:
Independent Antibody Validation in Cell lines (Figure 2) shows similar LSD1 expression profile in human and mouse cell lines detected by two independent anti-LSD1 antibodies that recognize different epitopes, 3761 against N-terminus domain and 14-050 against recombinant fusion protein. LSD1 proteins are detected in the most tested cell lines that include mouse (3T3/NIH) and rat cell lines (YB2/0) at different expression levels by the two independent antibodies.
- ISOFORMS: Human LSD1 has 2 isoforms, including isoform 1 (852aa, 93kD) and isoform 2 (876aa, 95kD). Mouse LSD1 has one isoform (853aa, 93kD) and Rat LSD1 also has one isoform (872aa, 95kD). 3761 can detect all isoforms of human, mouse and rat.
Properties
- PURIFICATION: LSD1 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: LSD1 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: LSD1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: KDM1
- ADDITIONAL NAMES: LSD1 Antibody: AOF2, KDM1, LSD1, BHC110, AOF2, KIAA0601, Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110
- Protein Accession Number: NP_001009999
- PROTEIN GI NUMBER: 58761544
- NCBI GENE ID NUMBER: 23028
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- LSD1 Antibody: Histone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins. LSD1 is a recently discovered amine oxidase that catalyzes the lysine-specific demethylation of histone proteins via an FAD-dependent oxidative reaction. Methylation on histone H3-K9 is thought to play an important role in heterochromatin formation, while methylation on arginine and some lysine residues (such as H3-K4) is associated with active transcription. LSD1 associates with various proteins, including HDAC1/2, CoREST, and BHC80, that act to regulate LSD1 activity in vivo, and in a histone H3-K4-specific methylase complex that is involved in transcriptional regulation. Experiments have shown that CoREST, a SANT domain-containing corepressor acts to enhance LSD1 activity, while BHC80, a PHD domain-containing protein, inhibits CoREST/LSD1 activity in vitro. LSD1-mediated histone demethylation thus may have significant effects on gene expression.
- 1: Shi et al. Cell 2004; 119:941-53.
- 2: Kouzarides et al. Curr. Opin. Genet. Dev. 2002; 12:198-209.
- 3: Shi et al. Mol. Cell 2005; 19:857-64.
- 4: Nakamura et al. Mol. Cell 2002; 10:1119-28.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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