- Trial Size
LNPEP Antibody
CATALOG NUMBER: 7055- Clonality: Polyclonal
- Tested Applications: E, IF, WB
- Host Species: Rabbit
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Mouse: (73%), Rat: (73%)
- IMMUNOGEN: LNPEP antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human LNPEP.
The immunogen is located within amino acids 900 - 950 of LNPEP. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, WB
- APPLICATION NOTE: LNPEP antibody can be used for detection of LNPEP by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. - SPECIFICITY: At least three isoforms are known to exist; this antibody will detect all three isoforms. Despite its predicted molecular weight, LNPEP often migrates at 165kD in SDS-PAGE.
- POSITIVE CONTROL 1: Cat. No. 1302 - Human Lung Tissue Lysate
- POSITIVE CONTROL 2: Cat. No. 10-101 - Human Lung Tissue Slide
- PREDICTED MOLECULAR WEIGHT: 113 kDa
Properties
- PURIFICATION: LNPEP Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: LNPEP Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: LNPEP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: LNPEP
- ADDITIONAL NAMES: LNPEP Antibody: CAP, IRAP, PLAP, P-LAP, OTASE, Leucyl-cystinyl aminopeptidase, Insulin-regulated membrane aminopeptidase, Cystinyl aminopeptidase
- Protein Accession Number: NP_005566
- PROTEIN GI NUMBER: 61742777
- NCBI GENE ID NUMBER: 4012
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- LNPEP Antibody: LNPEP, also known as insulin-responsive aminopeptidase (IRAP), was initially identified as an abundant protein that is associated with specialized endosomes termed GLUT4 storage vesicles (GSVs) in adipocytes. The luminal domain of LNPEP contains an aminopeptidase activity that is involved in peptide hormone processing and antigenic peptide processing. LNPEP is also a key regulator of GLUT4 trafficking by controlling the sorting of GLUT4 from endosomes to the GSVs.
- 1: Keller SR, Scott HM, Mastick CC, et al. Cloning and characterization of a novel insulin-regulated membrane aminopeptidase from Glut4 vesicles. J. Biol. Chem. 1995; 270:23612-8.
- 2: Wallis MG, Lankford MF, and Keller SR. Vasopressin is a physiological substrate for the insulin-regulated aminopeptidase IRAP. Am. J. Physiol. Endocrinol. Metab. 2007; 293:E1092-102.
- 3: Saveanu L, Carroll O, Weimershaus M, et al. IRAP identifies an endosomal compartment required for MHC class I cross-presentation. Science 2009; 325:213-7.
- 4: Jordens I, Molle D, Xiong W, et al. Insulin-regulated aminopeptidase is a key regulator of GLUT4 trafficking by controlling the sorting of GLUT4 from endosomes to specialized insulin-regulated vesicles. Mol. Biol. Cell 2010; 21:2034-44.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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