- Trial Size
LKB1 Antibody
CATALOG NUMBER: 3987- Clonality: Polyclonal
- Tested Applications: E, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rat: (100%), Chicken: (87%)
- IMMUNOGEN: LKB1 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human LKB1.
The immunogen is located within amino acids 310 - 360 of LKB1. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, WB
- APPLICATION NOTE: LKB1 antibody can be used for detection of LKB1 by Western blot at 1 μg/mL. LKB1 often migrates at a higher than expected molecular weight in SDS-PAGE.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. - POSITIVE CONTROL 1: Cat. No. 1216 - PC-3 Cell Lysate
Properties
- PURIFICATION: LKB1 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: LKB1 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: LKB1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: STK11
- ADDITIONAL NAMES: LKB1 Antibody: PJS, LKB1, hLKB1, PJS, Liver kinase B1
- Protein Accession Number: AAH19334
- PROTEIN GI NUMBER: 17939640
- NCBI GENE ID NUMBER: 6794
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- LKB1 Antibody: The LKB1 serine/threonine protein kinase was initially identified as a tumor suppressor gene mutated in human Peutz-Jeghers syndrome (PJS), a condition resulting in the growth of numerous intestinal polyps classed as hamartomas. LKB1 exists as a heterotrimeric complex with two other proteins, Ste20-related adaptor protein (STRAD) and MO25. Together, this complex can phsophorylate and activate the AMP-activate protein kinase (AMPK). Following AMPK activation by LKB1, AMPK then phosphorylates TSC1 and TSC2, key components of the metabolism-regulating TOR signaling pathway, which antagonizes the activation for the TOR pathway. LKB1 has also been shown to play a fundamental role in controlling the spatial orientation of structures required to maintain an ordered, polarized epithelium.
- 1: Hemminki A, Markie D, Tomlinson I, et al. A serine/threonine kinase gene defective in Peutz-Jeghers syndrome. Nature 1998; 391:184-7.
- 2: Boudeau J, Baas AF, Deak M, et al. MO25α/β interact with STRADα/β enhancing their ability to bind, activate and localize LKB1 in the cytoplasm. EMBO J. 2003; 22:5102-14.
- 3: Hawley SA, Boudeau J, Reid JL, et al. Complexes between the LKB1 tumor suppressor, STRADα/β and MO25α/β are upstream kinases in the AMP-activated protein kinase cascade. J. Biol. 2003; 2:28
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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