Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Listeria sp.
- IMMUNOGEN: Recombinant Listeria spp. p60.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, WB
- APPLICATION NOTE: ELISA: (direct and indirect: 1:5,000-1:10,000). Western Blot: (1:2,000-1:10,000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
- SPECIFICITY: Recognizes a wide range of Listeria p60 proteins.
Properties
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
- CONCENTRATION: 1 mg/ml
- STORAGE CONDITIONS: Stable for at least 6 months after receipt when stored at -20°C.
Additional Info
- NCBI OFFICIAL SYMBOL: iap
- ADDITIONAL NAMES: IAP p60 from Listeria Monocytogenes; Invasion-associated Protein p60 from Listeria Monocytogenes
- Protein Accession Number: 1171970
- PROTEIN GI NUMBER: P21171
- NCBI GENE ID NUMBER: 985140
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Listeria monocytogenes is a intracellular bacterium that is the causative agent of Listeriosis. It is one of the most virulent foodborne pathogens. Listeria species are known to secrete a 60-kDa protein collectively termed p60, which is encoded by the "iap" (invasion-associated-protein) gene and secreted in large quantites into the growth media. p60 is a highly immunogenic murein hydrolase that is essential for cell division.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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