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- SPECIES: Human, Mouse
- SOURCE SPECIES: E. coli
- RECOMBINANT PROTEIN SEQUENCE: Human LIF (aa 23-202) is fused at the N-terminus to a tag.
- FUSION TAG: GST tag
- APPLICATION NOTE: This recombinant proteins is for research use only.
- BIOLOGICAL ACTIVITY: Maintains pluripotency of mouse and human embryonic stem cells. The LIF (GST-tagged) from AdipoGen has the same activity as cleaved LIF.
Properties
- PURITY: >95% (SDS-PAGE).
Endotoxin level is less than 0.05EU/ μg purified protein (LAL test; Lonza). - PHYSICAL STATE: Lyophilized
- BUFFER: Contains PBS. Reconstitute with 100 μl sterile water.
- CONCENTRATION: 0.1mg/ml after reconstitution
- STORAGE CONDITIONS: Stable for at least 6 months after receipt when stored at -20°C.
Additional Info
- NCBI OFFICIAL SYMBOL: LIF
- ADDITIONAL NAMES: Leukemia Inhibitory Factor, Differentiation-stimulating Factor, D Factor, Melanoma-derived LPL Inhibitor
- Protein Accession Number: P15018
- PROTEIN GI NUMBER: 4504991
- NCBI GENE ID NUMBER: 3976
Background
- LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities also include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation and the stimulation of acute-phase protein synthesis in hepatocytes. LIF activates JAK & STAT signaling in human embryonic stem (ES) cells, but this pathway does not maintain pluripotency in these cells, which instead rely on FGF2-mediated ERK signaling. By contrast, mouse ES cells can be maintained by LIF-mediated JAK & STAT signaling. LIF binds to a high affinity heterodimeric receptor complex consisting of two proteins: LIF-R alpha that binds LIF with low affinity and the 130kDa (gp130) subunit that by itself does not bind LIF, but is required for high affinity binding of LIF.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
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