- HOST SPECIES: Mouse
- SPECIES REACTIVITY: Human
- IMMUNOGEN: LAG-3 antibody was raised against the extracellular domain of human LAG-3
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, Flow, ICC
- APPLICATION NOTE: LAG-3 antibody can be used for ELISA starting at 0.25 μg/mL. For Immunocytochemistry start at 1 μg/mL. For Flow Cytometry start at 1 μg/ml.
Antibody validated: Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested.
- POSITIVE CONTROL 1: Cat. No. 1331 - Human Lymphoma Tissue Lysate
- POSITIVE CONTROL 2: Cat. No. 1306 - Human Spleen Tissue
- PURIFICATION: LAG-3 Antibody is supplied as protein A purified IgG2a.
- CLONALITY: Monoclonal
- ISOTYPE: IgG2a,k
- PHYSICAL STATE: Liquid
- BUFFER: LAG-3 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: LAG-3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- NCBI OFFICIAL SYMBOL: LAG3
- ADDITIONAL NAMES: LAG3 Antibody: lymphocyte activating 3, LAG-3, CD223
- Protein Accession Number: NP_002277
- PROTEIN GI NUMBER: 167614500
- NCBI GENE ID NUMBER: 3902
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
- The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4). LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy.
- 1: Huard B, Tournier M, Hercend T, et al. Lymphocyte-activation gene 3/major histocompatibility complex class II interaction modulates the antigenic response of CD4+ T lymphocytes. Eur J Immunol 1994; 24:3216–21.
- 2: Triebel F. LAG-3: a regulator of T-cell and DC responses and its use in therapeutic vaccination. Trends Immunol 2003; 24:619–22.
- 3: Workman CJ, Wang Y, El Kasmi KC, et al. LAG-3 regulates plasmacytoid dendritic cell homeostasis. J Immunol. 2009; 182:1885–91.
- 4: Workman CJ, Vignali DA. The CD4-related molecule, LAG-3 (CD223), regulates the expansion of activated T cells. Eur J Immunol 2003; 33:970–9.
- FOR RESEARCH USE ONLY
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- Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
CATALOG NUMBER: RF16083
- Size: 0.02 mg | 0.1 mg
- List Price: $129.00 – $439.00
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